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251. The brain microvascular endothelial cell lines were seeded on collagen-coated Falcon tissue culture inserts in the top chamber‏‎ (2 versions)
252. The cells harvested with 100L Passive Lysis Buffer Reporter after 24 hours of treatment with EGF (or PBS) were subjected to reporter assays using the Dual-Luciferase Assay reporter system‏‎ (2 versions)
253. The cells were then washed three times with PBS and incubated with Texas red-conjugated secondary antibody (Santa Cruz Biotechnology) at a dilution‏‎ (2 versions)
254. The cellular concentration of heme is depending on the equilibrium between expression of hemoproteins, heme synthesis and heme degradation catalyzed‏‎ (2 versions)
255. The classification system provides information on the candidates regarding their molecular function and the biological processes and signal transduction pathways to which they belong‏‎ (2 versions)
256. The coding nucleotide sequence of the single-chain -FTase fusion construct is given in S1 File‏‎ (2 versions)
257. The coding sequences of annexins A1 and A6 were cloned into the Living Colours Fluorescent protein vectors yellow-fluorescent protein‏‎ (2 versions)
258. The control calibrator was a pool of reverse transcribed samples with each sample being normalized for internal control GAPDH‏‎ (2 versions)
259. The crystal structure of GRFT suggests that the 3 glycan-binding pockets of every GRFT monomer are on opposite ends in the double prism homodimer‏‎ (2 versions)
260. The cytotoxic effect of Huaier extract combined with 3-MA and CQ treatment was measured by MTT‏‎ (2 versions)
261. The data show that the control gene expression used to define altered gene expression in either condition is comparable‏‎ (2 versions)
262. The demographic variation of glaucoma in Denmark. The incidence and prevalence for 2011 is calculated‏‎ (2 versions)
263. The development of carboplatin-resistance is a major factor hampering the successful treatment of ovarian cancer with standard chemotherapy (carboplatin paclitaxel)‏‎ (2 versions)
264. The difference of required sample sizes was less pronounced when the power analysis was conducted with the data from the 10replicate experiment conducted during the short-day period of the year‏‎ (2 versions)
265. The effects on the demands of combat around the mental overall health‏‎ (2 versions)
266. The elution was repeated once, and the fractions were pooled. DTT was added to 5 mM, and the mixture was incubated at 37uC for 45 min‏‎ (2 versions)
267. The energy of this initial structure was minimized with 3,000 steps of conjugated gradient minimization using the CHARMM27 force field‏‎ (2 versions)
268. The enhanced phosphorylation of Mpk1 in cdc48-3 at high temperature suggests that the heat stress may be exacerbated in cdc48-3 mutant‏‎ (2 versions)
269. The expression of Beclin 1, the mammalian homolog of yeast autophagy gene Atg6 which is critical for induction of autophagy, is decreased in many cancer tissues and cancer cell lines‏‎ (2 versions)
270. The expression of ecto-59NT/CD73 and ADA is inversely regulated by interactions between b-catenin and TCF/LEF, resulting in a nuclear protein complex which interacts with the promoter region of these genes‏‎ (2 versions)
271. The extent of intracellular ROS levels are illustrated as intensity of green fluorescence observed in cells treated with no drugs‏‎ (2 versions)
272. The fact that muscle-specific or musclerelated miRNAs are highly enriched indicated that the data extracted from the duck breast muscle are credible‏‎ (2 versions)
273. The fact that normal cells and immortalized cells failed to respond to acid gradient indicates that the traditional ATP synthesis pathways like glycolysis‏‎ (2 versions)
274. The films were scanned and the optical density of each band, normalized to that of b-actin, was analyzed by using the public domain National Institutes of Health Image J software‏‎ (2 versions)
275. The findings suggest that ECFC tubule formation in the presence of FBS is, at least in part, VDR dependent‏‎ (2 versions)
276. The flow cytometry data indicated that IGF-IR is autophosphorylated in a higher percentage of brain-seeking cells, and that the mean intensity‏‎ (2 versions)
277. The germinal body contains nucleic acids and that germ cell determinant capability is lost when the germ plasm is irradiated with short-wavelength UV‏‎ (2 versions)
278. The gp41 subunit is a transmembrane protein composed of an ectodomain, a single membrane-spanning domain (MSD) and a cytoplasmic domain‏‎ (2 versions)
279. The hES cell-derived neurons exposed to NGF during differentiation exhibited significant increases in the number of cells responding to both ACh‏‎ (2 versions)
280. The histogram indicates the ratio of Phospho-Stat3 band intensities normalized to Stat3. Quantification of band intensity was performed with the Quantity One software‏‎ (2 versions)
281. The human urine samples used in this study were obtained as part of public health diagnostic activities and stored at CIETUS‏‎ (2 versions)
282. The identified proteins were functionally analyzed and grouped by using Uniport database‏‎ (2 versions)
283. The impact of glyphosate therapy on GR crops.‏‎ (2 versions)
284. The increase in protein yield may be due to a reduction in the rate of degradation or, alternatively, to an increase in the rate of protein synthesis‏‎ (2 versions)
285. The inhibitor was used with the appropriate controls according to the kit instructions provided by the manufacturer‏‎ (2 versions)
286. The initial model in the apo form of R1a was prepared by removing the coordinates of cAMP from the coordinate file‏‎ (2 versions)
287. The labelled transcripts were subjected to limited chemical and enzymatic probing prior to analysis on denaturing polyacrylamide gels‏‎ (2 versions)
288. The liver orchestrates a host defense response by altering (reprioritizing) the synthesis and systemic release of ``acute phase proteins''‏‎ (2 versions)
289. The lumen side of the SI was secured under a cover glass with a thin film of Vetbond‏‎ (2 versions)
290. The main difference Between Hair Wax and Herbal hair gel‏‎ (2 versions)
291. The maintenance of centromere stability is thus essential to prevent chromosomal instability and cancer development‏‎ (2 versions)
292. The methylation of the telomeric and centromeric 11p15 Imprinting Center Regions, termed ICR1 and ICR2, respectively, were analyzed by allele-specific methylated multiplex real time quantitative PCR (ASMM RTQ-PCR) as described previously‏‎ (2 versions)
293. The mitotic spindle is an essential component of cell division that ensures an equal division of the chromosomes‏‎ (2 versions)
294. The mode of drug administration imposes another limitation of the established pipeline, as penetration to inner organs can be hampered‏‎ (2 versions)
295. The most effective Rhinoplasty Originates from a Top Cosmetic surgeon‏‎ (2 versions)
296. The most famous Cellular phone Brands‏‎ (2 versions)
297. The most thermo-sensitive of all, MRC5 cell line, however, did not show increased Caspase 9 levels suggesting death by independent‏‎ (2 versions)
298. The negative control used was a 10-fold concentrated serum-free medium of untransfected cells. Supernatants were incubated with 100 nM 3H-ligand for 2 h at 37uC‏‎ (2 versions)
299. The number of vessels was counted in the whole section of each animal. Images were recorded with a confocal microscope‏‎ (2 versions)
300. The numerous Rewards of Meditation‏‎ (2 versions)

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