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Page créée avec « , Tokyo, Japan). Capillaries were [http://www.selleckchem.com/products/CAL-101.html mTOR inhibitor] quantified manually by a single individual from the digital image of in... »

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, Tokyo, Japan). Capillaries were [http://www.selleckchem.com/products/CAL-101.html mTOR inhibitor] quantified manually by a single individual from the digital image of individual fibres. The following indices were measured: (1) the number of capillaries around a fibre (NCAF); (2) the capillary-to-fibre ratio on an individual-fibre basis (C/Fi); and (3) capillary density (CD), which was calculated by using the fibre area as the reference space (Hepple & Mathieu-Costello, 2001). Capillary-to-fibre perimeter exchange index (CFPE) was calculated as an estimate of the capillary-to-fibre surface area (Hepple, 1997). Quantification of the capillary supply was performed on at least 50 fibres (Porter et al. 2002) by randomly selecting a fibre in an artifact-free region (no holes due to freeze fracture and fibres intact). Fibre cross-sectional area (FCSA) and perimeter (FP) was measured with the image analysis system and commercial software (SigmaScan Pro version 5.0; Systat Software, Inc., Point Richmond, CA, USA). All data are presented as means ��s.e.m. Separate one-way ANOVAs were performed to compare the relevant group means for each dependent variable. To examine body mass, a 2 �� 3 [time (pretraining and post-training) �� group (control, trained and day 7 detrained)] mixed factorial ANOVA was performed. To examine the VEGF protein response at rest and following a single 1 h exercise work bout, separate 2 �� 3 [condition (basal or exercise) �� group (control, trained and day 7 detrained)] factorial ANOVA for the plantaris and soleus muscles were performed. When appropriate, Tukey's [http://www.selleckchem.com/small-molecule-compound-libraries.html Small molecule library] post hoc HSD was used to determine which means were significantly different from each other (Keppel & Wickens, 2004). An �� level at P�� 0.05 was selected for all statistical comparisons. The analyses were conducted using the Statistical Package for the Social Sciences software (version 15.0, SPSS Inc., Chicago, IL, USA). The 2 �� 3 mixed factorial ANOVA revealed a significant time �� group interaction [F(2,30) = 69.97, P [http://en.wikipedia.org/wiki/Ficain Ficain] differences among groups for the two time points. As shown in Table 1, these analyses indicated no differences in body mass among groups for the pretraining time point [F(2,30) = 0.89, P= 0.42], but differences at the post-training time point [F(2,30) = 83.9, P

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Scenecold6 : Page créée avec « , Tokyo, Japan). Capillaries were [http://www.selleckchem.com/products/CAL-101.html mTOR inhibitor] quantified manually by a single individual from the digital image of in... »