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Blocks were finished with #600, 1000, 1500, and 2000 waterproof abrasive paper (Fuji Star, Sankyo, Rikagaku, Okegawa, Japan). The hardness of residual dentin was evaluated [https://en.wikipedia.org/wiki/Ergoloid Ergoloid] using Vickers microhardness testing machine (DongGuanSinowon precision instruments, Nancheng, DongGuan, China). The Vickers diamond indenter was applied to the dentin surface at 10N, and a dwelling time of 10 s was used for 10 indentations across the specimens of each group, resulting in a total of 50 indentations for all the groups. Five healthy dentin blocks were used as a positive control to contrast with the other groups under the same conditions. Data were recorded in Vickers microhardness (VHN). Micro-RAMAN analysis Dentin blocks were analyzed after caries removal with conventional rotatory instruments and chemomechanical system. Healthy dentin blocks were [http://www.selleckchem.com/products/CP-673451.html CP-673451 clinical trial] used as positive control. To clean the specimen for both groups and positive control, all samples from each group were ultrasonically cleansed in distilled water for 5 minutes and dried out at room temperature. Then, the samples were adhered to aluminum stubs. Blocks were analyzed using themicro-RAMAN system (HR-800; JobinYvon Inc., Edison, NJ, USA) equipped with a Microscope Olympus (BX-41) and a CCD as detector; the system is equipped with He-Ne laser (emission at 632.8 nm) at a power level of 60 ��W. Statistical analysis All experimental procedures were carried out by one blind investigator, the statistical analysis were performed for another author (Single blind). All variables were subject to Kolmogorov-Smirnov normality test. Demineralized teeth were analyzed using Wilcoxon signed rank test. Chemomechanical and conventional caries removal were evaluated with continuity correction (Yates correction) and odds ratio. McNemar test was used to compare incubation time between groups. Vickers microhardness of the remaining dentin was analyzed using ANOVA post-hoc Tukey test, and in case of micro-RAMAN analysis, data were tested using Spearman correlation. All data were analyzed with SPSS (Version 18; SPSS, Inc., Chicago, IL, USA). RESULTS In vitro caries model Molars in contact with [http://www.selleckchem.com/pharmacological_MAPK.html p38 MAPK activity] demineralized solution showed the following DIAGNO dent values: At 0 hour (55.17 �� 18.68), 120 hours (55.80 �� 28.01), and 240 hours (63.01 �� 29.92); Wilcoxon signed rank test showed significant differences in degree of demineralization. Values were tested using DIAGNOdent at 240 hours of incubation with solution; when comparing 0 hour (P

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Scenecold6 : Page créée avec « Blocks were finished with #600, 1000, 1500, and 2000 waterproof abrasive paper (Fuji Star, Sankyo, Rikagaku, Okegawa, Japan). The hardness of residual dentin was evaluated... »