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Page créée avec « After 24?h, 20??l of the incubated fluid was added to a MH agar plate and incubated at 37��C for 24?h. After 24?h, the CFUs were counted. The experiments were carried ... »

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After 24?h, 20??l of the incubated fluid was added to a MH agar plate and incubated at 37��C for 24?h. After 24?h, the CFUs were counted. The experiments were carried out in duplicate. The difference in the biofilm recovery after treatment with bioactive glass between time steps was analyzed with Freedman tests. The difference among all samples was calculated using ANOVA [https://en.wikipedia.org/wiki/BML-190 BML-190] and Games-Howell as post hoc tests. Statistical analyses were carried out using SPSS 20 (IBM, Armonk, NY). Prism 5 for Windows (GraphPad Software, Inc., La Jolla, CA) was used to create the graphs. The discs treated with BAG-S53P4 (0.5�C0.8?mm) and BAG-S53P4 ([http://www.selleckchem.com/products/DAPT-GSI-IX.html Selleckchem DAPT] kept the pH at 8 during all intervals (Fig. 2). The activity of bioactive glass against S. aureus planktonic cells showed 8 CFU log10 for BAG-S53P4 (0.5�C0.8?mm) and 8 CFU log10 for glass beads, while the CFU counting for BAG-S53P4 ([http://www.selleckchem.com/products/Adriamycin.html selleck] the effectiveness of this bone substitute against biofilm growth. The suppression rate of biofilm cells by BAG-S53P4

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Cloth59butter : Page créée avec « After 24?h, 20??l of the incubated fluid was added to a MH agar plate and incubated at 37��C for 24?h. After 24?h, the CFUs were counted. The experiments were carried ... »