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SC). Although nuclear staining (Fig. 3, arrowheads) of TDP43 became stronger (Figs. 3, 5B, left), cytoplasmic staining (Fig. 3, arrows) of TDP43 became evident from 1 day, with a peak at 1 month (Fig. 5B, left, blue bars) after the forebrain ischemia from a single 2-min tCCAO, which then gradually returned [http://www.selleckchem.com/products/BKM-120.html BKM120 in vivo] until 6 months. Typical cytoplasmic staining is shown in magnified insets in Figure 3. Among total TDP43-positive cells, the ratios of cytoplasmic positive pyramidal neurons were 1.8?��?1.3%, 2.7?��?1.2%, 5.2?��?2.4%, 10.8?��?3.1%, 7.1?��?2.5% and 6.6?��?3.3% at 1 day, 3 days, 7 days, 1 months (���P?[http://www.selleckchem.com/products/pexidartinib-plx3397.html this website] TDP43-positive cells, the ratios of cytoplasmic-positive pyramidal neurons were 15.1?��?7.5%, 32.6?��?10.6%, 24.5?��?10.3%, 21.8?��?9.0%, 22.7?��?9.5%, and 16.5?��?7.2% at 1, 3 (���P?[http://en.wikipedia.org/wiki/GUCY1B3 GUCY1B3] cells/mm2 at 1 day (**P?