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Whether cytokine level measurements can be as an indicator to guide the earlier discontinuation of antibiotic use is now under investigation. This study was supported in part by Grants from the National Natural Scientific Fund of China (Nos.?30170391 and 30971283), the Zhejiang Provincial Fund of Natural Science (No.?Z205166), and the Zhejiang Provincial Fund of Science and Technology Bureau (No.?2007C23007). The authors would also like to thank C. Li at Prince of Wales [http://www.selleckchem.com/products/mi-773-sar405838.html check details] Hospital, Chinese University of Hong Kong, for his critical comments and helpful suggestions regarding the manuscript. The authors thank H. Shen, B. Qian, C. Luo and N. Zhao at the haematology-oncology laboratory for their excellent technical support. None of the authors has any financial relationships with BD Biosciences/Pharmingen. ""The misuse of antibiotics has been related to increased morbidity, mortality and bacterial resistance. The development of antimicrobial stewardship programmes (ASPs) has been encouraged by scientific societies as an essential measure. An educational, institutionally supported ASP was developed in our tertiary-care centre. Local guidelines on the management of infectious syndromes were created. Antimicrobial prescriptions [https://en.wikipedia.org/wiki/Otenabant Otenabant] were chosen arbitrarily weekly and counselling interviews by expert clinicians were carried out, using a paedagogic, non-restrictive methodology. Satisfaction [http://www.selleckchem.com/products/lee011.html Ribociclib order] with the interview was assessed using anonymous questionnaires. The appropriateness of antimicrobial prescriptions as well as consumption was assessed prospectively throughout the year. Feedback regarding the correct use of treatments was communicated to each participating department periodically. The improvement in antimicrobial prescription was included among the annual objectives linked to economic incentives in every department. A total of 1206 counselling interviews were carried out during the first year. Fifty-three per cent of antimicrobial prescriptions (176/332) were inappropriate when the programme started. The rate of inappropriate prescriptions continuously declined to 26.4% (107/405) in the fourth trimester (p?